pgl3 basic luciferase reporter plasmid (Sangon Biotech)
86
Structured Review
Sangon Biotech
pgl3 basic luciferase reporter plasmid
Pgl3 Basic Luciferase Reporter Plasmid, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+plasmid/assay+bca+kit+protein/pmc12910405-316-13-18
Average 86 stars, based on 1 article reviews
Pgl3 Basic Luciferase Reporter Plasmid, supplied by Sangon Biotech, used in various techniques. Bioz Stars score: 86/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/pgl3-basic+plasmid/assay+bca+kit+protein/pmc12910405-316-13-18
Average 86 stars, based on 1 article reviews
pgl3 basic luciferase reporter plasmid - by Bioz Stars,
2026-10
86/100 stars
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Related Articles
Plasmid Preparation:Article Title: ACSL5 promotes lipid deposition and lipoapoptosis in proximal tubular epithelial cells of diabetic kidney disease. Article Snippet: Background: Lipoapoptosis in Proximal tubular epithelial cells (PTCs) are substantial in the etiology of diabetic kidney disease (DKD), yet the underlying mechanisms warrant further investigation.. Acyl-CoA synthetase longchain family member 5 (ACSL5) facilitates the formation of acyl-CoA, however, the precise role of ACSL5 in lipoapoptosis of PTCs in DKD remains inconclusive.. Methods: Transcriptomic data analysis identified the hub gene Acsl5 associated with lipid metabolism in DKD. Article Title: Blockade of endothelial adenosine receptor 2A suppresses atherosclerosis in vivo through inhibiting CREB-ALK5-mediated endothelial to mesenchymal transition Article Snippet: Cells were seed in 96-well plates and transfected with 100 ng constructed reporter plasmids using Lipofectamine 2000 (#11668019, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. .. To construct the ALK5 promoter reporter plasmid, a 2143 bp fragment of the ALK5 gene promoter (−1960/+183 relative to the transcription start site) was amplified using PCR and then inserted into the Article Title: SIX2 ‐Mediated Microglial M2 Polarization and Exosomal miR ‐3470b Delivery Protect Dopaminergic Neurons in Parkinson's Disease Article Snippet: The Dual‐LumiTM Luciferase Reporter Gene Assay kit was obtained from Beyotime Biotechnology (Shanghai, China). .. The promoter region of the mouse DDIT4 and GREM1 gene was subcloned into Luciferase:Article Title: ACSL5 promotes lipid deposition and lipoapoptosis in proximal tubular epithelial cells of diabetic kidney disease. Article Snippet: Background: Lipoapoptosis in Proximal tubular epithelial cells (PTCs) are substantial in the etiology of diabetic kidney disease (DKD), yet the underlying mechanisms warrant further investigation.. Acyl-CoA synthetase longchain family member 5 (ACSL5) facilitates the formation of acyl-CoA, however, the precise role of ACSL5 in lipoapoptosis of PTCs in DKD remains inconclusive.. Methods: Transcriptomic data analysis identified the hub gene Acsl5 associated with lipid metabolism in DKD. Article Title: miR-93-5p/IFNAR1 axis promotes gastric cancer metastasis through activating the STAT3 signaling pathway. Article Snippet: Aberrant expression of microRNAs (miRNAs) plays an important role in gastric cancer (GC) development. miR-93-5p has shown opposing functions in different types of cancers, but the exact expression pattern and molecular mechanism of miR-93-5p in GC development remain to be elucidated.. Here, we reported that miR-93-5p expression was increased in GC tissues compared with the adjacent normal tissues and that its overexpression was correlated with distant metastasis and poor survival in GC patients. miR-935p knockdown inhibited the migration, invasion and proliferation of GC cells in vitro and in vivo, while its overexpression displayed an opposite result.. Using an mRNA microarray, we found that miR-93-5p significantly downregulated IFNAR1 expression in GC cells, which was further identified as a direct target of miR-93-5p. Article Title: Melatonin attenuates low shear stress-induced pyroptosis and endothelial cell dysfunction via the RORα/miR-223/STAT-3 signalling pathway Article Snippet: .. Article Title: SIX2 ‐Mediated Microglial M2 Polarization and Exosomal miR ‐3470b Delivery Protect Dopaminergic Neurons in Parkinson's Disease Article Snippet: The Dual‐LumiTM Luciferase Reporter Gene Assay kit was obtained from Beyotime Biotechnology (Shanghai, China). .. The promoter region of the mouse DDIT4 and GREM1 gene was subcloned into Reporter Assay:Article Title: miR-93-5p/IFNAR1 axis promotes gastric cancer metastasis through activating the STAT3 signaling pathway. Article Snippet: Aberrant expression of microRNAs (miRNAs) plays an important role in gastric cancer (GC) development. miR-93-5p has shown opposing functions in different types of cancers, but the exact expression pattern and molecular mechanism of miR-93-5p in GC development remain to be elucidated.. Here, we reported that miR-93-5p expression was increased in GC tissues compared with the adjacent normal tissues and that its overexpression was correlated with distant metastasis and poor survival in GC patients. miR-935p knockdown inhibited the migration, invasion and proliferation of GC cells in vitro and in vivo, while its overexpression displayed an opposite result.. Using an mRNA microarray, we found that miR-93-5p significantly downregulated IFNAR1 expression in GC cells, which was further identified as a direct target of miR-93-5p. Mutagenesis:Article Title: miR-93-5p/IFNAR1 axis promotes gastric cancer metastasis through activating the STAT3 signaling pathway. Article Snippet: Aberrant expression of microRNAs (miRNAs) plays an important role in gastric cancer (GC) development. miR-93-5p has shown opposing functions in different types of cancers, but the exact expression pattern and molecular mechanism of miR-93-5p in GC development remain to be elucidated.. Here, we reported that miR-93-5p expression was increased in GC tissues compared with the adjacent normal tissues and that its overexpression was correlated with distant metastasis and poor survival in GC patients. miR-935p knockdown inhibited the migration, invasion and proliferation of GC cells in vitro and in vivo, while its overexpression displayed an opposite result.. Using an mRNA microarray, we found that miR-93-5p significantly downregulated IFNAR1 expression in GC cells, which was further identified as a direct target of miR-93-5p. Synthesized:Article Title: miR-93-5p/IFNAR1 axis promotes gastric cancer metastasis through activating the STAT3 signaling pathway. Article Snippet: Aberrant expression of microRNAs (miRNAs) plays an important role in gastric cancer (GC) development. miR-93-5p has shown opposing functions in different types of cancers, but the exact expression pattern and molecular mechanism of miR-93-5p in GC development remain to be elucidated.. Here, we reported that miR-93-5p expression was increased in GC tissues compared with the adjacent normal tissues and that its overexpression was correlated with distant metastasis and poor survival in GC patients. miR-935p knockdown inhibited the migration, invasion and proliferation of GC cells in vitro and in vivo, while its overexpression displayed an opposite result.. Using an mRNA microarray, we found that miR-93-5p significantly downregulated IFNAR1 expression in GC cells, which was further identified as a direct target of miR-93-5p. Construct:Article Title: Melatonin attenuates low shear stress-induced pyroptosis and endothelial cell dysfunction via the RORα/miR-223/STAT-3 signalling pathway Article Snippet: .. Article Title: Blockade of endothelial adenosine receptor 2A suppresses atherosclerosis in vivo through inhibiting CREB-ALK5-mediated endothelial to mesenchymal transition Article Snippet: Cells were seed in 96-well plates and transfected with 100 ng constructed reporter plasmids using Lipofectamine 2000 (#11668019, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. .. To construct the ALK5 promoter reporter plasmid, a 2143 bp fragment of the ALK5 gene promoter (−1960/+183 relative to the transcription start site) was amplified using PCR and then inserted into the Amplification:Article Title: Blockade of endothelial adenosine receptor 2A suppresses atherosclerosis in vivo through inhibiting CREB-ALK5-mediated endothelial to mesenchymal transition Article Snippet: Cells were seed in 96-well plates and transfected with 100 ng constructed reporter plasmids using Lipofectamine 2000 (#11668019, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. .. To construct the ALK5 promoter reporter plasmid, a 2143 bp fragment of the ALK5 gene promoter (−1960/+183 relative to the transcription start site) was amplified using PCR and then inserted into the Polymerase Chain Reaction:Article Title: Blockade of endothelial adenosine receptor 2A suppresses atherosclerosis in vivo through inhibiting CREB-ALK5-mediated endothelial to mesenchymal transition Article Snippet: Cells were seed in 96-well plates and transfected with 100 ng constructed reporter plasmids using Lipofectamine 2000 (#11668019, Thermo Fisher Scientific, USA) according to the manufacturer’s instructions. .. To construct the ALK5 promoter reporter plasmid, a 2143 bp fragment of the ALK5 gene promoter (−1960/+183 relative to the transcription start site) was amplified using PCR and then inserted into the |
